Evaluation of GNMT Gene Expression in Prostate Cancer Tissues using Real-Time PCR

Evaluation of gene expression level of GPRC6A, E.cadherin and ZEB1 in prostate cancer in comparison to benign tissues

Background: Prostate cancer is currently the third malignant disease in Iran and fifth common cancer worldwide. The aim of this study was to determine the expression of GPRC6A, E.cadherin, and ZEB1 genes in prostate cancer in comparison with benign tumor. Since early detection of cancer plays an important role in treatment, this study aims to identify the role of GPRC6A, E.cadherin and ZEB1 gen...

Study of the role of HOTAIR gene on colorectal cancer using Real-time PCR

BBackground: Despite advances in cancer studies, colorectal cancer, as the third most common cancer, has the highest mortality rate worldwide. Due to its high prevalence in the younger ages and advanced stages, screening of this cancer with molecular methods is necessary. Studies have shown that HOTAIR gene plays an important role in cancers. Our aim in the present study was to determine the...

Comparison of seminal vesicle, non-malignant and malignant prostate tissues with gene expression patterns using quantitative real-time PCR.

Investigation of marA Efflux Pump Gene Expression in Ciprofloxacin Resistant Salmonella enteritidis Clinical Strains Using Real-Time PCR

Background and Aim: Ciprofloxacin-resistant Salmonella spp. are among the most important causative agents of food-borne infections. The marA efflux pump in this bacterium plays a significant role in the development of drug resistance. The aim of this study was to investigate marA efflux pump gene expression in Ciprofloxacin resistant Salmonella enteritidis clinical strains by Real Time PCR. Mat...

Expression Analysis of Interferon Beta Level in HEK293T Cells Using Real-Time PCR and Protein Tests

Background: Interferons are some kind of natural cytokines which express in response to a variety of antigens including viral RNA, bacterial products, and tumor proteins. Interferon beta is used in the treatment of autoimmune diseases such as multiple sclerosis. Moreover, this drug inhibits cellular proliferation as well as angiogenesis and as a result, helps to cure cancer. In this research, i...

Determination of ctxAB expression in Vibrio cholerae Classical and El Tor strains using Real-Time PCR

Cholera is an infection of the small intestines caused by the bacterium V. cholerae. It is a major cause of health threat and also a major cause of death worldwide and especially in developing countries. The major virulence factor produced by V. cholerae during infection is the cholera toxin. Total mRNA extraction and reverse transcription was performed for making ctxAB cDNA. Relative Real-Time...